Open access
Aug 2026
A standardized method for T cell receptor (TCR) replacement through CRISPR-Cas9 mediated editing and retroviral transduction of primary murine naïve CD8 T cells
A protocol that combines ribonucleoprotein-based CRISPR-Cas9 editing with retroviral transduction to enable efficient genetic manipulation of murine CD8 T cells is described and it is shown that T cells engineered via this protocol can be generated at sufficient scale for downstream in vitro assays and in vivo adoptive transfer experiments.
Nadine Tong, John Attanasio, Eric Fagerberg et al.
· bioRxiv · 0 citations