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Sponsored AI Networking Cookbook: Practical recipes for AI-assisted network automation and development AI Networking Cookbook: Practical recipes for AI-assisted network automation and development

Author

Nadine Tong

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Open access Aug 2026

A standardized method for T cell receptor (TCR) replacement through CRISPR-Cas9 mediated editing and retroviral transduction of primary murine naïve CD8 T cells

A protocol that combines ribonucleoprotein-based CRISPR-Cas9 editing with retroviral transduction to enable efficient genetic manipulation of murine CD8 T cells is described and it is shown that T cells engineered via this protocol can be generated at sufficient scale for downstream in vitro assays and in vivo adoptive transfer experiments.

Nadine Tong, John Attanasio, Eric Fagerberg et al. · 0 citations

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