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N. Tleumbetova

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Open access Sep 2026

ПОЛУЧЕНИЕ РАСТВОРИМОГО РЕКОМБИНАНТНОГО AG85B MYCOBACTERIUM TUBERCULOSIS С ПОМОЩЬЮ ЭНДОГЕННОГО ПРОТЕОЛИТИЧЕСКОГО УДАЛЕНИЯ N-КОНЦЕВОГО ТИОРЕДОКСИНА В E. COLI

Recombinant expression of Mycobacterium tuberculosis antigen 85B (Ag85B) in Escherichia coli is frequently limited by poor solubility and accumulation of the target protein in inclusion bodies. Fusion to thioredoxin (Trx) can improve solubility; however, subsequent removal of the fusion partner using commercial proteas...

N. Tleumbetova, Ж. Ж. Марат, Л. А. Ибрагимова et al. · 0 citations

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