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The findings identify TSPAN3 as a marker of a previously unrecognized secretory MVB population distinguished by its intracellular trafficking, molecular interactions, and EV composition, supporting a model in which distinct tetraspanin-organized membrane nanodomains are associated with different intracellular trafficki...
How cells balance these competing routes using a genome-wide CRISPR activation screen for factors that alter surface expression of CD63-an EV-associated tetraspanin that shuttles between the PM and endosomes is investigated.
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