Open access
Aug 2026
STEP-PTMs: Sequential TMT-based Enrichment and Profiling of Post-Translational Modifications
Application of STEP-PTM to TMT16-plex cerebral brain organoids enabled the quantification of 10,413 proteins, 2,969 metabolites, 19,655 phosphopeptides, 28,876 peptides containing reversibly modified cysteines, 9,723 peptides containing free cysteines, 1,716 intact sialylated N-linked glycopeptides and 771 lysine-acetylated peptides from the same biological samples.
Lucrezia Criscuolo, S. B. Elmkvist, Arkadiusz Nawrocki et al.
· bioRxiv · 0 citations