Novel bioactive film (AP/P@LF/RCA) with integrated preservative and freshness‐indicating functionalities was prepared via a facile casting method. Apple pectin (AP) was loaded with lactoferrin‐phloretin nanoparticles (P@LF, acting as antibacterial agents) and red cabbage anthocyanins (RCA, serving as colour indicators). Structural characterization confirmed that P@LF and RCA formed a stable composite system with the AP matrix via hydrogen bonding interactions, which improved the crystallinity and thermal stability of the AP film. The film's water contact angle increased from 46.87° to 76.62°, boosting hydrophobicity. It exhibited 40.67% maximum elongation at break and 8.00‐MPa tensile strength, with effectively improved mechanical properties. Antibacterial assays confirmed that the film exhibited excellent antibacterial activity against both
Staphylococcus aureus
and
Escherichia coli
. The film achieved substantial degradation within 15 days. In the preservation of pork and cheese, the AP/P@LF/RCA‐0.15 film delayed the increase in pH, inhibited the growth of colonies and enabled real‐time visual monitoring of the freshness of pork and cheese through distinct colour change. Meanwhile, the film extended the shelf life of pork and cheese by 4 and 2 days, respectively. This study provides a convenient and nondestructive method for real‐time monitoring of food quality and exhibits great application potential in food preservation.
Lina Wei, Bingheng Li, Huating Yang et al.· Packaging technology & scien...· 0 citations
Background: Colorectal cancer (CRC) remains one of the leading causes of cancer-related mortality worldwide. Increasing evidence suggests that intestinal microbial dysbiosis contributes to colorectal tumorigenesis by reshaping host molecular signaling and the tumor immune microenvironment. However, the molecular mechanisms linking microbiome-associated alterations to host regulatory networks and disease progression remain incompletely understood. Objective: This study aimed to identify the microbiome-associated molecular regulators and immune modulators involved in colorectal cancer through an integrative multi-omics systems biology approach. Methods: Publicly available transcriptomic datasets were analyzed to identify differentially expressed genes, followed by functional enrichment, protein-protein interaction network construction, hub gene prioritization, immune infiltration profiling, survival analysis, and multi-omics characterization. Results: The identified hub genes represent hypothesis-generating host candidates for further mechanistic and clinical validation. These genes occupied key positions within host regulatory networks and were significantly associated with adverse clinical outcomes and altered CD8+ T-cell infiltration, suggesting their involvement in immune remodeling within the tumor microenvironment. Multi-omics characterization demonstrated that PTEN and SMAD4 alterations were predominantly associated with genomic deletions, whereas SMAD2 dysregulation was associated with transcriptomic and gene-dosage variation. Although the overall mutational burden of the identified hub genes was not significantly associated with disease-free survival (p = 0.878), these molecular regulators showed associations with immune-cell infiltration and CRC-related pathways. Conclusions: Collectively, our findings provide a system-level framework describing the associations between dysbiosis-relevant host pathways, CRC-related regulatory networks, and immune responses.
Huda Altoukhi, Nawal H. Siddig, N. Al-Hoshani et al.· Journal of Clinical Medicine· 0 citations
Acetoin is a valuable by-product of glucose metabolism with wide applications in the chemical, food, cosmetic, and pharmaceutical industries. This study investigated acetoin production using both wild-type and mutant strains of Bacillus paralicheniformis MT039446.1. Production was optimized by varying incubation time to determine conditions that maximized yield. UV-visible spectroscopic analysis showed distinct absororption peaks at 350 nm for the wild strain and 450 nm for the resistant mutant strain. Functional group characterization was further validated using Fourier transform infrared analysis confirmed the presence of carbonyl compounds. Structural and morphological changes in the solid substrate (press mud) during fermentation were examined through scanning electron microscopy and X-ray diffraction techniques, indicating substrate modification during microbial activity. Kinetic analysis demonstrated a significant improvement in the mutant strain (NA-cys3), with volumetric productivity (Qp) reaching 0.33 g/L·h compared to 0.16 g/L·h in the wild strain. This enhancement reflects improved efficiency of the acetoin biosynthetic pathway following mutagenesis. Overall, the study highlights an environmentally sustainable and economically viable approach for acetoin production using agro-industrial waste. This strategy supports the development of circular bio-economy models and offers potential for scalable bio-manufacturing applications.
Sikander Ali, M. Ahmad, Maryam Naz et al.· BioResources· 0 citations
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