TdGASA2, a cysteine-rich Snakin/GASA protein from Triticum turgidum, enhances stress-mediated inactivation of Listeria monocytogenes and improves the preservation performance of chitosan-based biodegradable active packaging films.
Results identify TdGASA2 as a promising, multifunctional bioactive candidate for biodegradable active food packaging; direct spectroscopic confirmation of the proposed protein-polymer interactions, migration behaviour in regulatory food simulants, toxicological and allergenicity profiling, sensory impact, and formal food-contact regulatory assessment remain to be established.