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Ming-Ming Yang

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Aug 2026

A novel biocontrol Pseudomonas species with broad-spectrum antagonistic activity against phytopathogens.

Bacterial and fungal diseases cause significant losses in horticultural crops, and biocontrol using beneficial microorganisms offers a sustainable alternative to chemical pesticides. In this study, a novel Pseudomonas strain D3 was isolated from Actinidiae rhizosphere. D3 exhibited strong antibacterial activity in LB medium but showed no activity against fungi or oomycetes. However, when cultured in KIDO medium, it demonstrated potent antifungal activity. Phylogenetic analysis based on 16S rRNA gene showed that D3 was most closely related to Pseudomonas mosselii CIP_105259T, while whole-genome sequencing revealed ANI values below 95% with eight known P. mosselii strains. Digital DNA-DNA hybridization (dDDH) further confirmed its genomic distinctiveness, with the highest dDDH value (58.2%) against the type strain P. mosselii DSM 17497T, well below the 70% species delineation threshold, supporting D3 as a novel Pseudomonas species. Functional validation via targeted gene knockout revealed a dichotomy in the antagonistic mechanisms of D3. Knockout of individual biosynthetic gene clusters (BGCs) only partially reduced antibacterial activity against Pseudomonas syringae pv. actinidiae, indicating that multiple BGCs contribute to this activity in a partially redundant manner. In contrast, disruption of a specific lipopeptide synthase cluster completely abolished antifungal activity against Valsa mali. LC-MS/MS analysis confirmed that this lipopeptide was produced exclusively in KIDO medium, consistent with the observed medium-dependent antifungal activity. Detached leaf and twig assays showed that D3 provides strong preventive biocontrol against both pathogens. Collectively, strain D3 employs a dual biocontrol mechanism, combining antibacterial activity mediated by multiple BGCs with lipopeptide-dependent antifungal activity, positioning it as a promising agent for sustainable disease management in horticultural crops.

Ming-Ming Yang, Guang-Zhe Li, Yun-Cong Wang et al. · Pesticide Biochemistry and P... · 0 citations

Open access Jul 2026

High Expression of CpMAPK9 Increased Jasmonic Acid Levels Potentially Improving Root Rot Resistance in Codonopsis pilosula

Background: This study aimed to investigate the molecular mechanisms underlying the response of Codonopsis pilosula roots to Fusarium oxysporum infection, which causes root rot and significant economic losses, and to provide a theoretical basis for breeding resistant varieties and developing effective control strategies. Methods: Root samples of C. pilosula were inoculated with F. oxysporum and harvested across five distinct intervals (0, 6, 24, 72, and 120 h after inoculation). Transcriptome sequencing was performed on 15 samples, generating 171.65 GB of clean data. De novo assembly was used to construct unigenes, followed by functional annotation and differential expression analysis. In addition, CpMAPK9 was transiently overexpressed in C. pilosula leaves to evaluate its role in jasmonic acid (JA) biosynthesis and disease resistance. Results: A total of 94,896 unigenes were obtained. Functional analysis showed that genes involved in hormone signal transduction, the MAPK signaling pathway, and plant–pathogen interactions were consistently activated in response to infection. Among the MAPK gene family, CpMAPK9 showed significant expression changes. Transient overexpression of CpMAPK9 significantly increased JA accumulation, indicating that CpMAPK9 positively regulates JA biosynthesis and may enhance resistance to root rot. Conclusions: In conclusion, our findings indicate that CpMAPK9 actively modulates JA-mediated defense pathways in C. pilosula during F. oxysporum invasion. Our findings shed light on the intricate molecular networks that drive disease defense, offering valuable theoretical insights to guide subsequent crop improvement initiatives.

Jia-Hao Cao, Yu-Fei Cheng, Yichuan Liang et al. · Genes · 0 citations