DNA helicases preserve genome stability by unwinding DNA during replication, repair, recombination, and transcription, yet their sites of action in vivo remain difficult to define. Here, we describe a sequencing-based strategy to map helicase activity genome-wide by coupling helicases to the single-stranded DNA-specifi...
Shaili Regmi, Noof Alsulaiti, D. Darling et al.· bioRxiv· 0 citations
Environmental surveys of wild yeasts typically rely on ribosomal barcodes, which cannot resolve cryptic species, interspecific gene flow, mixed cultures, or population structure. To determine what genome-scale characterization adds, we sequenced a representative panel of wild yeasts spanning the genera Saccharomyces, S...
Kennadi A. Shumaker, Kaitlyn Taylor, Spencer J. Gray et al.· bioRxiv· 0 citations
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