Efficient high-temperature simultaneous saccharification and fermentation of lignocellulosic substrates enabled by engineering thermotolerance in an enzyme-secreting industrial Saccharomyces cerevisiae strain.
The high cost of commercial enzyme cocktails remains a major barrier for lignocellulosic (second-generation) bioethanol production. Simultaneous saccharification and fermentation (SSF) at elevated temperatures using enzyme-secreting yeast can reduce enzyme demand, but is constrained by the limited thermotolerance of in...