Maja Jagodic

Whole-genome 5-hydroxymethylation profiling of human neurons for regulatory and biological insight

While 5-methylcytosine (5mC) DNA methylation is a well-known player in genome stability and transcriptional regulation, the role of 5-hydroxymethylcytosine (5hmC), particularly prevalent in neurons, remains largely unknown. Here, we used long-read Oxford Nanopore Technology (ONT) to profile whole-genome, native 5mC and...

Epigenetic mechanisms in neuroinflammatory disease of the brain: Multiple sclerosis.

DNA methylation landscape of cerebrospinal fluid cells in multiple sclerosis: an epigenome-wide association study

MS-associated methylation changes enriched in the cohesin chromatin-regulation pathway localised to T-cell regulatory regions included multiple protocadherin (PCDH) genes, which displayed consistent methylation and expression changes in CSF cells of pwMS compared to controls.

Single-cell analysis suggests coordinated immune-cell redistribution between blood and cerebrospinal fluid in early multiple sclerosis

Despite immune-cell infiltration being a hallmark of multiple sclerosis (MS), the interplay between the periphery and central nervous system immune responses is still incompletely characterized. We performed single-cell transcriptomic and V(D)J sequencing of paired blood and cerebrospinal fluid (CSF) immune cells from...