Open access
Jun 2026
Production of virus-like particles with AsCas12a nuclease and CMV-driven crRNA for mammalian genome editing
A detailed protocol is described for a small-scale production of AsCas12a-VLPs using three distinct transfection methods and a large-scale production of VLPs using calcium-phosphate transfection, showing that both production scales yield comparable nuclease loading into VLPs and similar editing efficiencies.
N. Kruglova, S. E. Borovikova, M. Shepelev
· Frontiers in Genome Editing · 0 citations