Unlocking the potential of Pleurotus ostreatus NRC 620 laccase isoenzymes in juice processing: Purification, kinetics, and biochemical characterization.
Two laccase isoenzymes, Lacc A and Lacc B, were purified and characterized from Pleurotus ostreatus NRC 620, and Lacc A was subsequently evaluated for apple juice treatment. The two isoenzymes exhibited distinct thermal profiles, where after 120 min, Lacc B retained about 92% and 82% of its activity at 40 and 50 °C, re...