ABSTRACT In this study, the enzyme inhibition and antioxidant capacity of Achillea biserrata were determined, and their relationship with cytotoxic effects was investigated. Antiproliferative activity was evaluated by the MTT assay in L929, A549, B16F10, Hep‐3B, PC‐3, MCF‐7, and CaCo‐2 cell lines, and enzyme inhibition was assessed against tyrosinase, urease, PTP‐1B, and DPP‐4. Ethanol extracts showed the greatest bioactivity, whereas water extracts were the least active. The capitulum ethanol extract (CEE) exhibited the strongest inhibition of tyrosinase, urease, DPP‐4, and PTP‐1B (IC50: 72.83, 40.84, 70.12, and 302.42 µg/mL, respectively) and the highest cytotoxicity (IC50: 168.42–325.42 µg/mL). LC‐MS/MS analysis identified quinic acid as the major constituent in the stem (44.614 mg/g extract) and capitulum (2.063 mg/g extract), and chlorogenic acid in the leaves (45.502 mg/g extract). Principal component analysis (PCA) integrating LC‐MS/MS and bioactivity data supported the bioactive potential of A. biserrata, highlighting its promise as a pharmaceutical resource.
Zühal Bayrakçeken Güven, M. A. Yılmaz, H. Inceer· Chemistry and Biodiversity· 0 citations
Inflammatory pain mediated by pro‐inflammatory factors remains a major clinical challenge, highlighting the need for safer alternatives to conventional anti‐inflammatory drugs. Here, the antinociceptive effects of methanolic extracts from the aerial parts of Alchemilla daghestanica (AD) and Alchemilla minusculiflora (AM) were evaluated in mice using the formalin test, and anti‐inflammatory activity was assessed by measuring formalin‐induced paw edema. Phytochemical characterization was performed by LC–MS/MS, and potential activities of major constituents were predicted using PASS Online. LC–MS/MS identified miquelianin as the predominant compound in both extracts. AD (100–200 mg/kg) and AM (200 mg/kg) significantly inhibited the inflammatory phase of the formalin test (p < 0.001 and p < 0.01, respectively) without affecting the neurogenic phase, and AM extract reduced paw edema (p < 0.05). PASS predicted high probabilities (Pa > 0.7) for anti‐inflammatory activity of miquelianin, isoquercitrin, and tannic acid. These findings suggest that these extracts may represent valuable candidates for further investigation in inflammatory pain management.
Elif Nur Barut, Belkıs Sadoğlu, Ayşenur Özduman et al.· Chemistry and Biodiversity· 0 citations
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