Development of an ERA-CRISPR/Cas12a-based visual diagnostic assay for bovine babesiosis
A novel diagnostic assay integrating enzymatic recombinase amplification (ERA) with CRISPR-Cas12a-mediated trans-cleavage, targeting the B. bovis spherical body protein 2 (SBP2) gene, providing a practical diagnostic tool for rapid, accurate detection of bovine babesiosis, with potential applicability to other veterina...