One-Pot Encapsulation of a Robust α-L-Rhamnosidase Mutant in Cu-MOF: A Recyclable Designed Biocatalyst for High-Yield Conversion of Icariin to Icariside I
Icariside I is a pharmacologically important prenylated flavonoid glycoside, yet its efficient biocatalytic production from icariin is hindered by the limited stability and recyclability of the enzymes involved. In this study, an α-L-rhamnosidase mutant R783A (Rha R783A) was heterologously expressed, purified, and immo...