Dynamic orchestration of structural and immune cells in acute Pseudomonas aeruginosa pneumonia revealed by time-resolved single-cell and spatial transcriptomics
Background Pseudomonas aeruginosa pneumonia triggers a complex, dynamic host response, yet the cellular coordination of inflammation and repair remains poorly defined. Methods We performed time-resolved single-cell RNA sequencing (scRNA-seq) on murine lungs across five timepoints (0–96 h post-infection) and integrated...