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Liang-Liang Sun

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Open access Aug 2026

A Draft Map of E. coli Proteoforms

An integrated multidimensional approach combining sequential protein extraction, size-exclusion chromatography (SEC) fractionation, and capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) and reversed-phase liquid chromatography (RPLC)-MS/MS is developed, offering strong evidence that MS-based TDP can...

Jorge A. Colón-Rosado, Fei Fang, Liang-Liang Sun · Analytical Chemistry · 0 citations

Open access Aug 2026

Proteoform Barcode: An Intuitive Visualization Framework for Top-Down Proteomics

Top-down proteomics (TDP) advances biomedical research by providing a bird’s-eye view of proteoforms in cells, tissues, and biofluids. Thousands of proteoforms can be characterized using well-established TDP technologies, and potential proteoform biomarkers of diseases have been discovered. However, there is a lack of...

Yifan Yue, Guangyao Gao, Fei Fang et al. · bioRxiv · 0 citations

2026

Combining SDS-PAGE with Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics Analysis of Intact Histone Proteoforms.

The combination of SDS-PAGE-based protein fractionation with CZE-tandem MS (MS/MS) for high-resolution characterization of histone proteoforms from a commercial calf thymus histone sample was described.

Fei Fang, Liang-Liang Sun · Methods in molecular biology · 0 citations