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Nanozyme-based biosensors have aroused much attention in recent decades and were broadly applied to multifarious areas. Whereas, the inferior reliability and high cost/complexity induced by the limited-modal outputs and commonly used multicomponent nanozymes in existing works are still drawbacks that cannot be ignored....
One-pot autocatalytic CRISPR biosensing offers a promising route for signal amplification without nucleic acid pre-amplification, but its efficiency is limited by an intrinsic readout-amplification conflict: the ssDNA reporter required for signal output competes with the autocatalytic mediator required for feedback amp...
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