Introduction The detection of tumor markers has become an important non-invasive detection method for early detection, diagnosis, and prognosis and treatment of tumors. Methods In this work, we designed and developed a novel type of electrochemical nano-biosensors characterized by magnetically driven self-assembly based on Fe3O4/α-Fe2O3 (ferriferrous oxide/α-iron trioxide, FOαIT) magnetic heterogeneous nanorods fabricated with CTAB as a restricted growth agent for sensitive detection of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1). Firstly, FOαIT@Au magnetic nanocomposites (MNCs) were prepared via the hydrothermal and calcination-reduction process with Au nanoparticles (AuNPs) modified on the surface. With the help of Au-S bonding, ssDNA/BC15 was immobilized to form FOαIT@Au/ssDNA/BC15. To avoid the effect of nonspecific sites to the detection, BSA was employed to block them and form FOαIT@Au/ssDNA/BC15/BSA nano-biosensors. Utilizing the stronger linkage force of hnRNP A1 with BC15 than that of FOαIT@Au/ssDNA, the sensitive and accurate detection of hnRNP A1 was realized. Results and Discussion The nano-biosensors revealed a decent linear relationship with a lower LOD of 0.103 pg/mL, higher sensitivity, excellent selectivity, satisfactory repeatability (RSD ≤1.55%), dependable long-term stability, and the accurate detection of spiked samples, revealing the promising application prospect in the clinical examination.
Lei Sun, Zhixiang Lv, Zhou Wang et al.· Frontiers in Chemistry· 0 citations
Hypoxia-inducible factor 1α (HIF-1α) protein has been identified as an aggressive malignant phenotype marker for numerous tumors; therefore, the detection of HIF-1α has become increasingly significant.
In this work, we developed a type of magnetically induced self-assembled electrochemical aptamer nanobiosensor for detecting HIF-1α protein. This nanobiosensor utilized the magnetism of hydrothermal-calcination-synthesized α-ferric oxide/ferriferrous oxide (α-Fe
2
O
3
/Fe
3
O
4
, αFO/FFO) magnetic heterogeneous nanorods (MHNRs) to realize magnetically induced self-assembly; further, gold nanoparticles (AuNPs) were used to reinforce the electron transfer capacity and realize secure immobilization of the aptamer via Au–S bonds. Finally, we adopted cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) for the electrochemical characterization, condition optimization, and performance analysis of the nanobiosensor, respectively.
The nanobiosensor revealed a favorable linear relation in the range of 0.1–1,000 ng/mL with a variance R
2
of 0.995 and limit of detection of 0.112 ng/mL, excellent sensitivity, faithful reproducibility with a relative standard deviation (RSD) of 2.28%, reliable 13-d stability with an RSD of 1.71%, and accredited detection capacity for real samples with recoveries of 97.71%–107.47% and RSDs ≤ 3.47%.
In this study, we developed a magnetically induced self-assembled electrochemical aptamer nanobiosensor for ultrasensitive detection of HIF-1α protein. The nanosensor delivers outstanding analytical performance with a facile assay strategy suitable for point-of-care testing, suggesting its promising prospects for clinical tests.
Xiangjun Zhou, Hezhong Ouyang, Li-Ping Sui et al.· Frontiers in Chemistry· 0 citations
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