L. M. Blank

Comparison of two genetic systems for protein production in Cupriavidus necator H16.

Many chemical manufacturing routes are being replaced with enzymatic processes to improve sustainability. In biocatalysis, enzyme production is often the main bottleneck. Bacterial proteins are frequently produced using the workhorse E. coli BL21(DE3) and its derivatives. However, other bacteria with beneficial charact...

FLP/FRT-Mediated Recombination for Resistance Marker Recycling.

Enhancing acetate-based biosurfactant production by Pseudomonas putida KT2440 through fermentation optimization

Background There is an increasing demand for sustainable chemicals, although “green premium” can be rarily achieved. In this context, biosurfactants emerge as promising substitutes for petroleum- or palm-oil-based surfactants due to their potentially lower environmental impact. 3-(3-hydroxyalkanoyloxy)alkanoic acids (H...

Improving acetate metabolism of P. putida KT2440 by evolutionary and rational engineering

Acetate is a promising carbon source for microbial biotechnology as it can be produced sustainably from lignocellulosic biomass or C1 gases. Since acetate is directly activated to acetyl-CoA, it is especially suitable for producing acetyl-CoA-derived products, showcased here with the production of 3-(3-hydroxyalkanoylo...

Adaptive laboratory evolution rewires Pseudomonas putida for resource-efficient acetate assimilation

Acetate is an attractive renewable two-carbon substrate for microbial biotechnology, but its toxicity limits growth and carbon-use efficiency at process-relevant concentrations. Here, we used adaptive laboratory evolution to improve acetate tolerance in a genome-reduced strain of Pseudomonas putida and combined whole-g...