Digital Quantification of Membrane DAB Immunohistochemical Staining in FFPE Cervical Cancer Tissues Using an Open-Source CellProfiler Pipeline
Immunohistochemistry (IHC) is a highly specific and widely used laboratory technique for assessing protein localization and expression in tissue samples. Interpretation of 3,3’ diamino benzidine (DAB)-based IHC is often based on observer-dependent manual scoring or traditional imaging software, which may show variabili...