Planococcus versutus sp. nov., isolated from soil
This dataset contains the digitized treatments in Plazi based on the original journal article See-Too, Wah-Seng, Ee, Robson, Madhaiyan, Munusamy, Kwon, Soon-Wo, Tan, Jia Yi, Lim, Yan Lue, Convey, Peter, Pearce, David A., Yin, Wai Fong, Chan, Kok-Gan (2017): Planococcus versutus sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology 67 (4): 944-950, DOI: 10.1099/ijsem.0.001721, URL: http://dx.doi.org/10.1099/ijsem.0.001721 Abstract A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15 T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4–30 Ǫ C, pH 6–11 and at moderately high salinity (0–15 %, w/v, NaCl), with optimal growth at 26 Ǫ C, at pH 7–8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15 T belonged to the genus Planococcus and was closely related to Planococcus halocryophilus Or 1 T (99.3 % similarity), Planococcus donghaensis JH 1 T (99.0 %), Planococcus antarcticus DSM 14505 T (98.3 %), Planococcus plakortidis AS /ASP6 (II) T (97.6 %), Planococcus maritimus TF-9 T (97.5 %), Planococcus salinarum ISL-6 T (97.5 %) and Planococcus kocurii NCIMB 629 T (97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1–81.7 % to the type strains of these closely related species of the genus Planococcus. The principal fatty acids were anteiso-C 15: 0, C 16: 1 Ɯ 7 c and anteiso-C 17: 0, and the major menaquinones of strain L10.15 T were MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15 T represents a novel species of the genus Planococcus, for which the name Planococcus versutus sp. nov. is proposed. The type strain is L10.15 T (= DSM 101994 T = KACC 18918 T). The genus Planococcus was proposed by Migula [1] to accommodate aerobic, Gram-stain-positive, motile, coccus- or rod-shaped bacteria. In 2001, five Planococcus species were transferred to the newly proposed genus Planomicrobium to differentiate rod-shaped, motile, nonsporogenous and low G+C content bacterial species within the original genus Planococcus [2]. These two genera can be differentiated through their 16S rRNA gene sequences, which were shown to have sequence signatures at positions 183 (T for Planococcus and C for Planomicrobium) and 190 (A for Planococcus and G for Planomicrobium), following the 16S rRNA gene sequence numbering of Escherichia coli. To date, according to the List of Prokaryotic Names with Standing in Nomenclature (LPSN) (www.bacterio.net/planococcus.html), there are 12 species described in the genus Planococcus. Although 18 species are cited in the files of the genus Planococcus in LPSN, six of these have been reclassified to the genera Planomicrobium or Marinococcus. Members of Planococcaceae are able to survive extreme environments having been isolated from a wide range of sources, including deep-sea sediments, marine solar salterns, glaciers, permafrost, Antarctic deserts, faeces, cyanobacterial mats and sea ice brine [3 – 6]. All members of the genus Planococcus are able to grow at moderately low temperatures (psychrotrophic) and are moderately halotolerent (halophilic). The type strain of Planococcus halocryophilus, which was isolated from Artic permafrost, was reported to grow and divide even at extremely low temperature (―15 Ǫ C) [7]. Members of the genus Planococcus can be exploited in the field of biotechnological and industrial applications, for instance through their production of carotenoids, thermophilic and alkaline/salt-tolerant xylanases and biosynthesis of butanol [3, 8 – 10]. Here, we provide a detailed taxonomic characterization of a novel member of the genus Planococcus, strain L10.15 T, which was recently isolated from Antarctic soil samples. In this study, strain L10.15 T was isolated during an ecological survey of the quorum-quenching (QQ) soil bacteria in Antarctic soil samples using QQ bacteria enrichment medium [11]. The soil sample was collected from an elephant seal wallow in Lagoon Island, close to Adelaide Island, off the west coast of the Antarctic Peninsula (67 Ǫ 35.689 ′ S 068 Ǫ 14.495 ′ E). Briefly, around 1 g of soil sample and 5 ml of sterile QQ bacteria enrichment medium with the sole carbon source of 100 µg synthetic N -hexanoyl-L- homoserine lactone (C 6 -HSL) were added to a sterile 50 ml polypropylene conical tube and incubated at 4 Ǫ C with agitation at 150 r.p.m. A total of 100 µl of the bacterial suspension was transferred into new QQ bacteria enrichment medium, including C 6 -HSL, after 1 week of incubation. This step was repeated three times, and finally, 100 µl of bacterial suspension was plated onto Luria–Bertani (LB) agar. An orange-pigmented isolate, strain L10.15 T, was recovered. The cell suspensions were kept in 20 % (w/v) glycerol stock for long-term storage at ―80 Ǫ C. Strain L10.15 T was then routinely cultured aerobically in LB broth or on LB agar at 26 Ǫ C (optimum growth temperature). As this is the first reported Planococcus species with QQ activity, we sequenced its complete genome using Pacific Biosciences RSII to facilitate our investigation. Colonies of strain L10.15 T were orange-pigmented, circular, entire, smooth, convex and 1–2 mm in size on LB agar after 48 h of incubation at 26 Ǫ C. Gram staining was performed using the Difco Gram stain set and observed using a Leica DM 750 microscope (Leica Microsystems). Cells of strain L10.15 T were Gram-stain-positive with no spore formation. Electron micrographs were obtained using a table top scanning electron microscope (TM3030; Hitachi) and a scanning transmission electron microscope (LIBRA 120; Carl Zeiss). For scanning electron microscopy, a sample was prepared as described by Vali et al. [12]. For scanning transmission electron microscopy, an overnight suspension of cells was stained using 1 % phosphotungstic acid on a Formvar grid and observed at an operating voltage of 80 kV. Cells of strain L10.15 T were coccoid, typically 1.0– 1.5 µm in diameter, mostly arranged as diplococci, but single cells or tetrads were also observed (Fig. 1). A catalase test was conducted using 3 % (v/v) H 2 O 2 and determined by observing the production of copious bubbles. Oxidase activity was determined using 1 % (w/v) N, N, N ′, N ′,-tetramethyl 1,4-phenylenediamine (bioḾerieux) as described by Smibert and Krieg [13]. API ZYM and Biolog GEN III microplates were prepared according to the manufacturers’ instructions. The activities of various enzymes were determined by using the API ZYM system after incubation for 24 h. Antibiotic susceptibility was tested by using ATB PSE 5 strips (bioḾerieux) and the disc diffusion assay following the manufacturer’ s instructions. All tests were performed at 26 Ǫ C and in triplicate. The temperature range for growth was determined by plating on LB agar and by incubating at 4–37 Ǫ C with increments of 1 or 2 Ǫ C for 14 days. The pH range for growth of strain L10.15 T was determined on LB agar plates adjusted to various pH values between 4 and 12 with 1 pH unit increments. Salt tolerance was determined by growing on LB agar media supplemented with 0–25 % (w/v) NaCl at increments of 1 %. Both salt tolerance and pH range tests were conducted by incubating the LB agar plates at 26 Ǫ C for up to 14 days. The results of physiological tests of strain L10.15 T as compared with closely related species are presented in Table 1. Genomic DNA of L10.15 T was extracted from an overnight cell suspension culture using the MasterPure Gram-positive DNA purification kit (Epicentre Technologies). A 20 kb SMRTbell template library was then constructed using the extracted genomic DNA. The whole genome sequencing was performed using Pacific Biosciences RSII sequencing platform with C4 chemistry in two single-molecule realtime cells. The complete genome of strain L10.15 T has been sequenced, enabling the discovery of the gene responsible for QQ activity [8]. To determine the identity of strain L10.15 T, the 16S rRNA partial gene sequence was amplified from the extracted DNA obtained as described above by using primers 27F and 1492R [14] and analysed using the Ex-Taxon database [15]. Pairwise similarity analysis demonstrated that strain L10.15 T is a member of the genus Planococcus, with P. halocryophilus Or 1 T (99.3 %), Planococcus donghaensis JH 1 T (99.0 %), Planococcus antarcticus DSM 14505 T (98.3 %), Planococcus plakortidis AS /ASP6 (II) T (97.6 %), Planococcus maritimus TF-9 T (97.5 %), Planococcus salinarum ISL-6 T (97.5 %) and Planococcus kocurii NCIMB 629 T (97.5 %) as the closest relatives present in the database. Phylogenetic analyses were conducted using the full 16S rRNA gene sequence (1538 bp) retrieved from the complete genome sequence. The MEGA 6.0 software [16] was used to perform the alignment using the MUSCLE algorithm [17], and the phylogenies were reconstructed using default settings of neighbour-joining (Fig. 2), maximum-likelihood (Fig. S1, available in the online Supplementary Material) and maximum-parsimony (Fig. S2) algorithms. The 16S rRNA gene sequence of L10.15 T contained the signature nucleotides of Planococcus, T and A, respectively, at positions 183 and 190 (E. coli 16S rRNA gene sequence numbering) and thus clustered separately from the related genus Planomicrobium [18]. All 16S rRNA gene phylogenies concordantly demonstrated that strain L10.15 T clustered within Planococcus but formed a distinct branch separate from P. halocryophilus Or 1 T, P. donghaensis JH 1 T, P. antarcticus DSM 14505 T, P. plakortidis AS /ASP