Foodborne diseases pose a severe threat to human health. As a critical meat-borne pathogen, Escherichia coli O157:H7 (E. coli O157:H7) can trigger severe illnesses such as hemorrhagic enteritis, threatening public safety and causing a huge economic impact on the meat industry. However, previous detection methods have struggled to strike an optimal balance among accuracy, reliability, and efficiency, with various traditional techniques exhibiting obvious technical shortcomings. To overcome these limitations, this study developed a novel biosensor for the rapid detection of E. coli O157:H7 based on bimetallic carbon-based nanozymes. This sensor leverages the superior peroxidase-like activity and excellent intrinsic fluorescence of bimetallic carbon dots, providing a solid foundation for constructing a dual-modal platform combining colorimetry and fluorescence. Ultimately, this collaborative approach enables the rapid and accurate detection of the pathogen, substantially enhancing the reliability and stability of the results with a limit of detection (LOD) of 10 cfu/mL. This research presents significant practical application value and offers an innovative design strategy for developing advanced diagnostic sensors for E. coli O157:H7 and other meat-borne pathogens.
Hongzhou Chen, Weichao Wu, Mengyu Li et al.· Foods· 0 citations
Staphylococcus aureus (S. aureus) is a significant pathogen that causes foodborne diseases. Meat, with its abundant nutrients and high water activity, constitutes an ideal niche for S. aureus colonization. Numerous studies have shown that human digestive tract diseases caused by consuming meat products contaminated with S. aureus occur frequently. It is of great significance to strictly monitor S. aureus in meat matrices. A novel biosensor employing dual-signal output was developed through the combination of efficient magnetic separation and dual-modal precise detection. Designed for the rapid enrichment of S. aureus, it significantly boosts detection sensitivity and accuracy, thereby enabling the earlier identification of potential contamination sources. This method uses vancomycin-modified magnetic beads as the capture element, and aptamer-modified nanozymes as the signal element. After magnetic separation, the 3,3′,5,5′-tetramethylbenzidine color reaction can be used to quickly and sensitively detect S. aureus. It achieved a detection limit as low as 10 cfu/mL. Moreover, this dual-signal sensor based on efficient magnetic separation can sensitively detect S. aureus in meat products, thus showing good application prospects in food matrices and further improving the reliability and specificity of detection.
Temperature-dependent variations in the molecular characteristics of intact spores, including changes associated with Ca2+-dipicolinic acid, protein-related, and nucleic acid-related spectral features under different sporulation temperatures are revealed.
Mengya Li, Kequan Xing, Sun-Hee Wang et al.· Food Research International· 0 citations
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