Characterization of METTL3/14-mediated m6A modification in human transcriptome using Nanopore direct RNA sequencing
This study leveraged ONT to comprehensively profile bona fide m6A modifications in cellular RNAs at single-nucleotide resolution by integrating two direct RNA sequencing chemistries with the m6Anet and Dorado modification-detection models and establishes a robust transcriptome-wide framework for RNA modification detect...