Author

K. Rasal

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Open access Jul 2026

Design and in silico validation of donor DNA for RNA-guided recombinase-mediated knockout of mstnb gene in Labeo rohita

It is suggested that the engineered mutation is likely to abolish the functional activity of the mstnb gene, thereby providing a validated donor DNA construct for precise RGR-mediated genome editing in L. rohita.

Lukram Sushil Singh, Porkodi Murugesan, Gowhar Iqbal et al. · PLoS ONE · 0 citations

Open access Jul 2026

Design and transfection of CRISPR/Cas9 constructs for the myostatin gene in Labeo rohita muscle cells

The study successfully established CRISPR/Cas9 plasmid constructs for the mstnb gene in L. rohita and demonstrated their transfection in LRDM cell line across multiple passages, providing a basis for future studies on genome editing approaches using CRISPR/Cas9 constructs in fish muscle cell lines.

Gowhar Iqbal, Nevil Pinto, Darshan Pawaskar et al. · Scientific Reports · 0 citations