Aptamer selection often yields multiple sequence families that recognize different epitopes or bind through distinct molecular interactions. Rather than using a single aptamer for detection, we demonstrate that these complementary aptamers can be integrated into a cross-reactive sensor array to enhance molecular disc...
Yun Shu, Pan-Ting Sang, Shi-Yuan Liu et al.· Analytical Chemistry· 0 citations
Green fluorescent protein (GFP) variants are established as a tractable model system for studying protein-binding aptamers using strategies commonly applied to small-molecule aptamers and provide a roadmap for achieving small-molecule-level mechanistic insight into protein-binding aptamers.
This work employed capture-SELEX, which uses free target molecules in solution without immobilization, to isolate DNA aptamers that specifically recognize doxorubicin and significantly inhibited doxorubicin uptake by HeLa cells, establishing a new generation of anthracycline aptamers.
Meheta Datta, Celine Fidella, Lashaun N. Coote et al.· ACS Chemical Biology· 0 citations
It is shown that commonly used modeling approaches, including RNAComposer and AlphaFold3, fail to reliably reproduce aptamer conformations, particularly at the binding sites critical for molecular recognition.
ThT fluorescence is a useful label-free method to study voriconazole aptamers and that aptamers can be used to probe target binding to nanoscale host molecules and that aptamers can be used to probe target binding to nanoscale host molecules.
