Joseph M. Dobbs

Single-cell visual proteomics of a minimal bacterium reveals structural coordination of gene expression machineries.

Cryo-electron tomography of native and antibiotic-perturbed Mycoplasma pneumoniae cells is used to resolve 140 maps that recapitulate bacterial translation during the initiation, elongation, and recycling phases, allowing us to propose a threading-based translation reinitiation mechanism and to provide structural evide...