Telomere to telomere genome assembly of a wild soybean Glycine soja accession HAAS216
Wild soybean ( Glycine soja ), the progenitor of cultivated soybean ( Glycine max ), represents an important reservoir of genetic diversity crucial for soybean improvement. However, the limited availability of high-quality reference genomes for wild soybean has constrained its comprehensive genomic characterization. Here, we present a telomere-to-telomere (T2T) genome assembly of a wild soybean ( G. soja ) accession HAAS216 with a size of 1,018.17 Mb, generated using PacBio HiFi, ultra-long Oxford Nanopore sequencing, and Hi-C data. The assembly spans all 20 chromosomes and captures 20 centromeric and 40 telomeric regions, forming a gap-free T2T genome assembly. This assembly exhibits superior quality regarding completeness (BUSCO: 99.6%), contiguity (contig N50: 51 Mb), and base-level accuracy (QV: 55.53). Repetitive sequences make up 55.84% (568.57 Mb) of the genome, and 48,390 protein-coding genes were predicted, of which 47,567 (98.30%) were functionally annotated. This genome assembly provides a genomic resource for comparative genomics and facilitates genetic research and crop improvement in soybean.