Elucidating Antibody-Hapten Recognition for 25-Hydroxyvitamin D3 toward a Point-of-Need Immunoassay.
Accurate quantification of 25-hydroxyvitamin D3 (25(OH)D3), the gold-standard biomarker for vitamin D nutritional assessment, still presents considerable analytical challenges. Although 25(OH)D3 exhibits intrinsic photosensitivity and thermal lability, these properties can be effectively managed via standardized light-...