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Author

Jing-Wen Zhou

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Sep 2026

Combinatorial optimization of ribosomal binding site, promoter and genomic locus for high-yield production of a thermostable transglutaminase in Streptomyces mobaraensis.

Streptomyces mobaraensis transglutaminase (smTG) is an important food-processing biocatalyst, yet its industrial potential is limited by low yield and thermal instability. Here, we report the multi-level engineering of S. mobaraensis smY2022 for high-level production of a thermostable variant, TGm2. Ab initio design of...

Xiao-Fei Hu, Jia-Cai Ye, Jing-Wen Zhou et al. · 0 citations
Sep 2026

Rational design of heptaprenyl diphosphate synthase and metabolic optimization drive biosynthesis of menaquinone-7 by Escherichia coli.

Menaquinone-7 (MK-7) is a fat-soluble vitamin with important nutritional and pharmaceutical applications. However, microbial biosynthesis of MK-7 is limited by low catalytic efficiency of key enzymes, insufficient precursor supply, and imbalanced availability of nicotinamide adenine dinucleotide phosphate (NADPH). In t...

Zi-Hao Li, Kang-Jie Xu, Yi-Yi Chen et al. · 0 citations

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