MS3 acquisition and μ-SPE cleanup enable sensitive quantification of PPG1000 in zebrafish tissues: systematic comparison with MRM and in-source CID strategies.
This work provides the first systematic comparison of MRM, MS3, and in-source CID for PPG quantification, establishing a sensitive analytical platform with complementary greenness and practical applicability assessments using AGREE, AGSA, CACI, and GEMAM, for polymer bioanalysis in complex matrices.