Amination of Epigallocatechin Gallate and Dihydromyricetin in a Caenorhabditis elegans Culture Medium Causes Experimental Artifacts by Reducing the Free Amino Acid Content.
Pyrogallol-type polyphenols undergo Strecker degradation with amino acids in cell culture media, yet whether such chemistry measurably alters the nutritional composition of Caenorhabditis elegans growth medium and affects worm phenotypes remains unclear. Using a tert-butyldimethylsilyl derivatization-based GC-MS workfl...