RPA-CRISPR/Cas12a Assay for ALV-J with Broad-Spectrum Detection and Reduced Residual Amplicon Load.
Avian leukosis virus subgroup J (ALV-J) is a retrovirus that poses a serious threat to the poultry industry. It is prone to genetic variation, leading to the emergence of multiple epidemic strains, which creates substantial challenges for clinical detection. Although the recombinase polymerase amplification (RPA)-CRISP...