A biosurfactant‐producing fungal strain, Exophiala spinifera CSF123, was isolated from cashew oil‐contaminated soil. Internal transcribed spacer sequencing followed by phylogenetic analysis revealed that the isolated fungus shared 98.42% similarity with E. spinifera CBS66.76. The biosurfactant molecule, named ES‐414, produced by the fungus was purified to homogeneity by HPLC, and subsequent electrospray mass spectrometric analysis showed that its neutral mass is 414 Da. The molecule displayed remarkable antibiofilm activity. At concentrations of 15 and 80 μg/mL, ES‐414 showed 62.17% biofilm inhibition against Candida tropicalis and 66% inhibition against Candida albicans, respectively. It also displayed significant antifungal properties against C. albicans, C. tropicalis, Candida glabrata, Candida parapsilosis, and Candida krusei. ES‐414, in a dose‐dependent manner, inhibited the quorum sensing activity of Chromobacterium violaceum. Thermogravimetric analysis of ES‐414 indicated that the molecule remains stable up to 240°C. Detailed structural characterization using GC‐MS, FTIR, UV‐visible spectroscopy, elemental analysis, Molisch test, TLC analysis, and solubility studies indicated that ES‐414 is a glycolipid‐like molecule.
Amit Yadav, Swathy Sadanandan Anand, B. G. Nair et al.· International Journal of Mic...· 0 citations
Marine-derived probiotic bacteria have emerged as promising alternatives to antibiotics in sustainable aquaculture owing to their resilience, metabolic versatility, and ability to produce beneficial bioactive compounds. In the present study, the probiotic and functional potential of
Bacilllus subtilis
ULB16, a marine endophytic bacterium isolated from
Ulva lactuca
, was comprehensively evaluated. The safety assessment revealed susceptibility to several clinically relevant antibiotics, while no extracellular DNase activity was detected, supporting its non-virulent nature. The strain exhibited tolerance to gastrointestinal-like stress conditions, including acidic pH, bile salts, and simulated gastric juice, indicating its ability to survive gastrointestinal transit. Functional probiotic characterization demonstrated cell surface hydrophobicity, auto-aggregation, and co-aggregation with representative enteric pathogens (
Escherichia coli
,
Salmonella enterica
, and
Klebsiella pneumoniae
) as well as aquaculture-associated pathogens (
Aeromonas hydrophila
and
Vibrio parahaemolyticus
).
B. subtilis
ULB16 also exhibited biofilm-forming ability and showed higher adhesion to HT-29 intestinal epithelial cells than the commercial probiotic strain
Alkalihalobacillus clausii.
Notably, the isolate exhibited β-galactosidase activity of 0.0565 U/mL, a relatively uncommon feature among
Bacillus
-based probiotics, suggesting additional nutritional and functional benefits associated with lactose metabolism. Collectively, the findings demonstrate that
B. subtilis
ULB16 possesses a combination of desirable probiotic traits, including stress tolerance, pathogen interaction capability, epithelial adhesion, and functional enzyme activity. These multifunctional properties highlight its potential as a promising marine-derived probiotic candidate for sustainable biotechnological applications.
Swathy Sadanandan Anand, Sreetha Hely, B. G. Nair et al.· Frontiers in Marine Science· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
