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3D-MAESTRO: A scalable, modular, portable pipeline for automated processing of large-scale volumetric brain microscopy data
3D-MAESTRO is applied to lightsheet images of whole mouse brains in the context of diverse anatomical and functional experiments, illustrating high-throughput and reproducible mapping of microscopic structures across the brain.
Functionally identified neuronal assemblies robustly encode stimuli and are structurally delineated by inhibition.
In vivo optical physiology with postmortem electron microscopy is combined to extract neuronal assemblies and shows that these assemblies can serve as functional units of perception and suggest they may be structurally delineated by mutual inhibition.