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Sponsored Artificial Intelligence: A Modern Approach, Global Edition Artificial Intelligence: A Modern Approach, Global Edition

Author

J. Rousseau

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Open access Sep 2026

Split Cas12a Protospacer Engineering Enables Ultraspecific Recognition of a PAM-Less DNA Strand via an External PAM-Proximal Duplex

CRISPR-Cas12a is a programmable RNA-guided endonuclease whose activation by double-stranded DNA normally depends on the recognition of an adjacent protospacer-adjacent motif (PAM), which constrains target selection in diagnostic applications. Here, we divide the DNA activator into PAM-proximal (Pp) and PAM-distal (Pd)...

G. Lamothe, Félix Veillette, I. Iwe et al. · 0 citations
Open access Aug 2026

Prime Editing Mediated Generation and Correction of the mdx5cv Mutation Restores Dystrophin Expression in Myoblasts

An in vitro prime editing workflow is established to generate and subsequently correct the mdx5cv mutation in mouse C2C12 myoblasts and provides proof of concept that precise correction of the pathogenic mutation is associated with restoration of dystrophin expression following myogenic differentiation.

A. Siddika, Fatima El Husseiny, J. Rousseau et al. · 0 citations

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