Ilias G. Karvounis

A dual gating mechanism controls target‐strand cleavage in Cas12j: Implications for engineering efficient nickases

These findings provide a mechanistic basis for tuning Cas12j activity along the nuclease‐to‐nickase spectrum, supporting the rational engineering of genome‐editing tools with controlled target‐strand cleavage kinetics, and offering a path toward applications that bypass dependence on Non‐Homologous End Joining (NHEJ or...