I. Alves

Real-time analysis of pore formation by bi-component staphylococcal leukotoxins using the two-electrode voltage-clamp technique

A simple and highly sensitive method allowing cell surface expression of a large diversity of target receptors and recording in real-time, currents generated by neo-formed pores is developed, able to elucidate the kinetics of pore formation, the limiting role of receptors in this process, and propose a complementary mo...