J -Aggregation-Reshaped pK a in a Spirocyclic BODIPY Enables Ratiometric Lysosomal pH Sensing and Super-Resolution Imaging
Ratiometric fluorescent pH probes typically rely on complex intramolecular energy/charge transfer, rendering the design strategies increasingly rigid. We report a conceptually distinct ratiometric probe based on pH-driven J-aggregate disassembly utilizing a spirocyclic BODIPY platform. Strategic installation of β-Cl...