Consensus design and in silico screening of GH42 β-galactosidase for enhanced catalytic hydrolysis of whey lactose.
This study addresses a major bottleneck in dairy waste management by engineering a highly efficient, thermophilic enzyme for lactose hydrolysis, transforming the cheese manufacturing by-product whey into a high-value resource. While traditional glycoside hydrolase family 2 (GH2) enzymes suffer from poor industrial ther...