The integrated CRISPR/Cas detection workflow from sample collection and processing through final result output is assessed, and the intrinsic characteristics of Cas effector proteins with respect to target enrichment, reporter molecules, readout formats, sample background, and validation design are analyzed.
Rong Gao, Hong-Li Jin, Tian-Yi Zhang et al.· Biotechnology Advances· 0 citations
This study reveals Snapin, a neuronal trafficking regulator, to be a critical host factor co-opted by RABV to enhance neuroinvasion and identifies a Snapin-dependent GSK3β/NF-κB axis as a potential therapeutic target for rabies treatment.
Yu-Jie Bai, Yong-Sai Liu, Xiao-Yu Wu et al.· Journal of Virology· 0 citations
A visual nucleic acid detection assay for SBV that combines reverse transcription recombinase‑aided amplification (RT‑RAA) with the CRISPR/Cas12a system, targeting conserved regions of the SBV S gene holds promise for further clinical validation, although its diagnostic utility in authentic clinical specimens remains t...