A Single-Tube Dual-Gene RAA-PfAgo Assay for Simultaneous Detection and Identification of PEDV Field and Vaccine Strains.
A one-tube visual detection platform based on recombinase‑aided amplification (RAA) and PfAgo nuclease for simultaneous detection of the highly conserved N gene and the virulence‑associated ORF3 gene of PEDV, offering a powerful tool for on-site clinical diagnosis, strain differentiation, and epidemiological monitoring...