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Hiroaki Takesue

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Open access Aug 2026

Strategic enhancement of Cas9 nickase-driven gene amplification.

This work develops a plasmid-based reporter system in budding yeast for the rapid identification of high-performing gRNAs in budding yeast and introduces BITREx 2.0, a dual-nicking strategy that targets both sides of the gene array.

Hiroaki Takesue, Satoshi Okada, Takashi Ito · Cell Reports Methods · 0 citations