Giuseppina Pisignano

mBACS enhances pseudouridine profiling through efficient and robust chemical conversion

Pseudouridine (Ψ) is the most abundant RNA modification and regulates RNA stability, splicing, and translation. Utilizing the dual nucleophilic N1 and O2 of Ψ, we previously developed 2-bromoacrylamide- assisted cyclization sequencing (BACS) for quantitative, single-base Ψ detection. Here, we screened new dual electrop...