Probiotics are indispensable functional microorganisms in food fermentation with increasingly promising application prospects. Although genetically modified probiotics show great potential in improving the quality of fermented foods, their practical application is hampered by ethical controversies and safety concerns....
Kai-Ge Peng, Gui-Fang Tian, Ke-Min Mao et al.· Food Research International· 1 citation
Escherichia coli is a dominant host for recombinant protein production due to its well-characterized genetics and low cultivation cost, yet high-level intracellular expression frequently leads to insoluble inclusion bodies. Targeting proteins to the periplasm represents an effective strategy for improving soluble expre...
Fruit pomace recycling is critical for green biomanufacturing. Lactococcus lactis, a common host for waste valorization, shows poor protein expression in conventional systems. We constructed T7RNAP-driven inducible and constitutive expression systems in L. lactis NZ9000. The two systems increased target gene transcript...
Kai-Ge Peng, Fang-Kun Zhao, Xuejiao Zhang et al.· Journal of Agricultural and...· 1 citation
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