Open access
Aug 2026
Easy detection of CRISPR/Cas9-Induced Insertions, Deletions, and Substitutions in Rice Genes OsMADS26, OsRAC1 and OsNRT1.1b using SYBR Green qPCR and Robust HRM analysis in R software.
An adapted HRM workflow combining conventional SYBR Green-based qPCR chemistry with downstream computational analysis to detect CRISPR/Cas9-induced mutations at three rice loci is evaluated, supporting the potential applicability of the approach for individual sample analysis and expanded sample screening.
Bianca Cristina Carvalho Reis, Céline Georget, Anne-Cécile Meunier et al.
· New Biotechnology · 0 citations