Open access
Sep 2026
Size and sequence variation of the Lid of the Cas12a nuclease domain results in a nickase phenotype
Abstract CRISPR–Cas12a nucleases typically induce double-strand breaks (DSBs) to initiate genome editing. However, reliance on DSBs risks chromosomal rearrangements and unintended insertions or deletions at off-target sites. Therefore, engineering nickase variants that cleave only a single DNA strand might enable preci...
Ricardo Villegas Warren, Satoshi N. Omura, Diana León-Castro et al.
· Nucleic Acids Research · 0 citations