Use of Green Fluorescent Protein (GFP) to illustrate intra- and extracellular expression of recombinant proteins in the yeast Pichia pastoris
At the Federal University of São Carlos (São Carlos, SP, Brazil), third-year biotechnology undergraduate students are required to take the theoretical and practical course "Expression and Purification of Recombinant Proteins”. The course includes laboratory exercises that show how to express proteins in the Pichia past...