Restoration of Spirulina growth in recycled medium via a combined strategy of nutrient supplementation and flocculation treatment.
Spirulina is recognized as a nutrient-dense superfood. Recirculation of cultivation medium offers a sustainable and cost-effective approach for microalgae production. However, the present study revealed that the Spirulina biomass yield in the second recycled medium (R2 batch) in shake flasks was 70.8% lower than that i...